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KMID : 0381120100320030267
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Genes and Genomics
2010 Volume.32 No. 3 p.267 ~ p.273
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Development and characterization of twenty-five new polymorphic microsatellite markers in proso millet (Panicum miliaceum L.)
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Cho Young-Il
Chung Jong-Wook
Lee Gi-An
Ma Kyung-Ho
Dixit Anupam
Gwag Jae-Gyun
Park Yong-Jin
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Abstract
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Millets such as proso millet have excellent nutritional properties and could become a basic resource for crop breeding programs and food diversification. In this study, 25 polymorphic microsatellite markers were developed and characterized through construction of an SSR-enriched library from genomic DNA of proso millet (Panicum miliaceum L.). In total, 110 alleles were detected, with an average of 4.4 alleles per locus. Values of major allele frequency (M AF ) and expected heterozygosity (H E ) ranged from 0.36 to 0.98 (mean = 0.73) and from 0.04 to 0.74 (mean = 0.37), respectively. The mean genetic similarity coefficient was 0.3711, indicating that among 50 accessions of proso millet there was wide genetic variation. The newly developed microsatellite markers should be useful tools for assessing genetic diversity, understanding population structure, and breeding of proso millet.
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KEYWORD
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Microsatellite markers, Panicum miliaceum L., Proso millet, SSR-enriched library
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